• C. C. Ojianwuna
  • Ebele Martina Ilondu
  • V. N. Enwemime
Keywords: Asteraceous plants, Culex quinquefasciatus, Efficacy, Larvicide, Leaf extracts


It is well known that the mosquito, Culex quinquefasciatus, badly affect individuals in endemic setting, causing filariasis. Intervention targeting the vector larva has not been given much priority. Therefore, the efficacy of ethanolic extract of Ageratum conyzoides, Bidens pilosa L. and Spilanthes filicaulis and a mixture of these plants in equal proportion was evaluated for larvicidal activities against filarial mosquito Culex quinquefasciantus. Phytochemical screening of the extracts was done by dissolving 5g each of plants in 30ml ethanol and water separately to obtain plant extracts. Samples were qualitatively screened following standard methodology for phytochemicals. Mortality of mosquito larva was examined after 12hrs, 24hr, 36hrs, and 48hrs treatment. Probit analysis for 50% Lethal Concentration was done using GraphPad Prism 9. Phytochemical screening of the plants revealed that Bidens pilos, Spilanthes filicaulis and Ageratum conyzoides contained moderated concentrations of Anthraquinones and Cardiac glycosids, Tannins, and Alkaloids respectively. Complete mortality was recorded with 0.60ml of Bidens pilosa, and Spilanthes filicaulis at 48hours and 36 hours respectively. More so, complete mortality was recorded with 0.40 and 0.60ml of the three plants in mixed proportion at 36 hours and 24 hours respectively. Larval mortality and time mortality were significantly (p<0.05) different. 50%Lethal Concentration for Ageratum conyzoides L, Bidens pilosa L, Spilanthes filicaulis, and mixture of the three plants in equal proportion are 0.33ml, 0.25ml, 0.08ml, and 0.06ml respectively. Results showed that these plant materials exhibit significant activity and could be considered as potent natural larvicidal agent.


Aarthi,N. and Murugan, K. (2010). Larvicidal and smoke repellent activities of Spathodea campanulata P. Beauv. Against the malarial vector Anopheles stephensi L. (Diptera: culicidae). Journal of Phytology, 2(8):61-69

Akobundu, I. O.and Agyakwa, C. W. (1998). A hand book of West African weeds.International Institute of Tropical Agriculture,pp 162-193

Allison, L.N., Dike, K.S, Opara, F.N., Ezike, M.N. and Amadi, A.N.(2013). Evaluation of larvicidal efficacy and phytochemical potential of some selected indigenous plant against Anopheles and Culex quinquefasciatus. Advance Bioscience and Biotechnology, 4:1128-1133.

Arivoli,S., and Samuel,T. (2001). Bioefficacy of Citrullus colocynthis (L) Schrad (Cucurbitaceae) whole plant extract against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). International Journal of Current Research 3(4):296-304

Arivoli,S., Samuel,T., and Jesudoss, M.J. (2011). Lavicidal efficacy of Vernonia cinerea (L) (Asteraceae) leaf extracts against filarial vector Culex quinquefasciatus Say (Diptera Culicidae). Journal of Biopesticides 4(1):37-42

Ashafa, A.O.T., and Afolayan, A.J. (2009). Screening the root extracts from Bidens pilosa L.var radiate (Asteraceae) for antimicrobial potentials. Journals of Medical Plants Research, 3(8): 568-572

Bairwa.K., Kumar.R., Sharma.R.J. and Roy.R.K. (2010). An updated review on Bidens pilosa L. Journal of Pharmacitical and Chemical Rresearch, 2(3):325-337

Bouda,H., Topondjo,A., Foptem,D.A and Gumedzoe,M.Y.D. (2001). Effect of essential oils from leaves of Ageratum conyzoides, Lantana camara and Chromolaena odorata on the mortality of Sitophilus zeamais (Coleoptera, Curculionidae). Journal of Stored Products Research, 37(2):103-109.

Chandra, G, Mondal, R.P, Singh, A, and Ghosh, A. (2016). Studies on larvicidal activities of some plant extracts against filarial vector Culex quinquefasciatus. Journal of Mosquito Research, 6(10): 53-76.

Chandra,F., Darriet,F., Darder,M., Cuany,A., Doannio,J.M.C., Pasteur,N., and Guillet, P. (1998). Pyrethroid resistance in Culex quinquefasciatus from West Africa. Journal of Medical Verterinary Enthomology, 12:359-366.

Chiang,Y., Chang,C.L., Chang,S., Yang,W., and Shyur,L. (2007). Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator. Journal of Ethnopharmacology,110:532-538

Coleman,M., Vontas,J.G., and Hemingway,J. (2002). Molecular characterization of the amplified aldehyde oxidase from iinsecticide resistant Culex quinquefasciatus. Journal of Biochemistry, 269:768-779

Das, N.G., Goswani D. and Rabha, B. (2007). Preliminary evaluation of mosquito larvicidal efficacy of plant extracts. Journal of Vector Borne Disease, 44:145-148

Evans,W.C and Trease,G.E. (1999). Phharmacognosy. W.B Sunders Company Limited, Notinghan,U.K pp 310-315.

Ilondu, E.M., Ojeifo, I.M., and Emosairue, S.O. (2014). Evaluation of antifungal properties of Ageratum conyzoides, Spilanthes filicaulis and Tithonia diversifolia leaf extracts and search for their compounds using gas chromatography- Mass Spectrum. ARPN Journal of Agricultural and Biological Science, 9(11): 375 – 384.

Iqbal J, Ishtiaq F, Alqarni AS, and Owayss AA. 2018. Evaluation of larvicidal efficacy of indigenous plant extracts against Culexquinquefasciatus (Say) under laboratory conditions. Turkish Journal of Agriculture and Forestery, 42: 207-215.

Islam, J., Zaman, K., Tyagi, V., Duarah, S., Dhiman, S. and Chattopadhyay, P. (2017). Protection against mosquito vectors Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus using a novel insect repellent, ethyl anthranilate. Acta Tropica 174: 56-63.

Kabaru,J.M., Gichia, L.(2001). Insecticidal activity of extracts derived from different parts of the mangrove tree Rhizophora mucronata (Rhizophoraceae) Lam. against three arthropods. African Journal of Science and Tecchnology, 2(2):44-49

Kalu, I.G., Eroegbusi J., Nwachukwu, C.U., and Ibeh, B. (2010). Larvicidal activities of ethanol extract of Allium sativum (garlic bulb) against the filarial vector, culex quinquefasciatus. Journal of Medicinal Plants Research 4(6):496-498

Kamaraj, C., Bagavan, A., Elango, G., Abduz, Zahir A., Rajakumar, G., Marimuthun,S., Santhosshkumar, T., and AbdulRahuman, A. (2009). Larvicidal activity of medicinal plant extract against anopheles subpictus and culex triitaeniorhynchus. Indian Journal of Medicinal Plants Research, 134:101-106

Kaushik, R. and Saini, P. (2008). Lavicidal activity of leaf extract of millingtonia hortensis (family: Bignoniaceae) against anopheles stephensi, culex quinquefasciatus and aedes aegypti. Journal of Vector Borne Disease, 45:66-69

Maheswaran, R., Sathish, S., and Ignacimuthu, S. (2008). Larvicidal activity of Leucas aspera (wild) against the larvae of culex quinquefasciatus say and aedes aegypti L. International Journal of Integrative Biology,2(3):214-217

Mohamed, R.A.H., Nagmouchi, S., Al-Keridis, L.A. and Benammar, R.(2020). Evidence based efficacy of selected herbal extracts against Culex quinquefasciatus (Say) Larvae. Pakistan Journal of Biological Sciences, 22(3): 127-132.

Prabhakar,K. and Jabanesan, A. (2004). Mosquitocidal effect of bitter gourd leaf extracts. Bioresource Journal of Technology, 95:113-114

Taylor MJ, Hoerauf A, Bockarie M. 2010. Lymphatic filariasis and onchocerciasis Lancet 376: 1175-1185.

WHO (2016). World malaria report 2016. World Health Organization, Geneva Switzerland.

World Health Organization, WHO (2010). Global Programme to eliminate lymphatic filariasis: progress report on mass drug administration in 2009. Wkly Epidemiology Rec, 85: 265-372.

World Health Organization, WHO (2011). Meeting of the international task force for disease eradication-October 2010. Wkly Epidemiology Rec, 86: 53-59.

World health organization (1996). Report of the WHO informal consultation on the evaluation on the testing of insecticides, Geneva: p69

WHO (2020). World malaria report 2016. World Health Organization, Geneva Switzerland.

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